Journal: Cell Death & Disease

Article Title: Clarithromycin inhibits autophagy in colorectal cancer by regulating the hERG1 potassium channel interaction with PI3K

doi: 10.1038/s41419-020-2349-8

Figure Lengend Snippet: a Effects of 5-FU on proliferation of HCT116 cells, alone (IC 25 : 2.2 µM; IC 50 : 13.7 µM) or in combination with Cla (IC 25 : 40 µM; IC 50 : 80 µM). Experiments were carried out as described for Fig. . Data points give the number of trypan blue-negative cells ( n = 3, each carried out in triplicate). Full statistics are reported in Table . b Percentages of apoptotic (early apoptotic plus late apoptotic from Annexin V/PI staining) HCT116 cells treated as in ( a ) for 24 and 48 h ( n = 3). c Time course of spheroids’ volume relative to their initial volume (time 0) for the indicated conditions. Drugs were added at their IC 50 and IC 25 values, obtained in 2D cultures ( n = 3; 8 samples for each condition). Spheroids’ photographs, taken with an inverted microscope (Nikon Eclipse TE300, with a ×10 objective), were used to calculate spheroid volumes as in ref. by SpheroidSizer1_0, a MATLAB-based and open-source software (MATLAB 2015a, MathWorks Inc.). The reported significance values refer to the comparison between each condition and control cells. d , e Volumes ( d ) and cell viability ( e ) of spheroids treated as in ( c ) for 120 h. Spheroid volumes are reported as the percentage of control ( n = 3; 8 samples for each condition). Cell viability was assessed by the live/dead imaging (see Materials and Methods) ( n = 2; 6 samples for each condition). f Representative 3D reconstruction of tumor masses, performed in B-Mode imaging with VevoLAZR-X (scale bar, 5 mm) (left panel). Time course of tumor growth relative to the volume determined at the beginning of treatment (day 7 after the inoculum) (right panel). One week after subcutaneous (s.c) inoculation (3 × 10 6 HCT116 cells/flank), mice were treated for two weeks with saline (control group), Cla (40 mg kg −1 , twice daily by oral gavage (o.g.), 5-FU (30 mg kg −1 , twice a week intraperitoneally (i.p.)), or the combination of Cla plus 5-FU ( n = 2 mice; n = 4 tumor masses, for each for each experimental group), as reported in the schematic representation of treatment regime. Statistical analysis was performed with one-way ANOVA: Cla + 5-FU vs. control mice, P = 0.019; Cla + 5-FU vs. Cla mice, P = 0.033; Cla + 5-FU vs. 5-FU mice, P = 0.046. g Expression of phospho-ERK1/2 Thr202/Tyr204 and pro-caspase 3 in tumor masses obtained from mice treated as in ( f ). Membranes were re-probed with anti-ERK1/2 or antitubulin antibodies, to normalize data. The corresponding densitometric analyses are given in the bar graphs ( n = 4 for each experimental group). h IHC analysis of hERG1 and p53 in tumor masses of HCT116 tumor xenografts of mice treated as in ( f ). Representative images are reported (original magnification, ×400; scale bar, 100 µm). The percentages of cells positive for either hERG1 or p53 are reported in the bar graph ( n = 2 mice, n = 4 tumor masses for each experimental group). Statistical significance was assessed with one-way ANOVA for ( a – h ); * P < 0.05; ** P < 0.01, and *** P < 0.001.

Article Snippet: Spheroids’ photographs, taken with an inverted microscope (Nikon Eclipse TE300, with a ×10 objective), were used to calculate spheroid volumes as in ref. by SpheroidSizer1_0, a MATLAB-based and open-source software (MATLAB 2015a, MathWorks Inc.).

Techniques: Staining, Inverted Microscopy, Software, Comparison, Control, Imaging, Saline, Expressing

Journal: Nature Communications

Article Title: In-situ visualization of solute-driven phase coexistence within individual nanorods

doi: 10.1038/s41467-018-04021-1

Figure Lengend Snippet: Gradual loading of Pd nanorods. a Loading states of four nanorods are shown at different hydrogen pressures. The color of the points shows the β-spectral contributions of the point spectra (calculated using MCR) collected at that position of the nanorod. The β-phase is indicated by red and α is indicated by blue. The scale bar is 20 nm for the first two columns and 100 nm for the last two. b 34 nanorods showing that nanorods shorter than 55 nm (with ~10 nm radii) tend not to support phase coexistence, whereas nanorods longer than 250 nm are more likely to support two phase boundaries

Article Snippet: To fit the EEL specta and spectral images, we use MCR based on ref. (open-source MATLAB package).

Techniques:

Journal: Nature Communications

Article Title: In-situ visualization of solute-driven phase coexistence within individual nanorods

doi: 10.1038/s41467-018-04021-1

Figure Lengend Snippet: Phase progression along the length of nanorods. a – d TEM images and EEL spectral images of four different nanorods at 100 K while cooling started from 254 Pa. The collected EEL spectral images have been decomposed into constituent spectra using MCR and the α-spectral and β-spectral weights are superimposed to produce the figures. The nanorod boundaries are drawn approximately as a guide to the eye. The scale bars correspond to 20 nm for ( a ) and 50 nm for ( b – d ), respectively in x -axis. Along y -axis, scale bar is 20 nm

Article Snippet: To fit the EEL specta and spectral images, we use MCR based on ref. (open-source MATLAB package).

Techniques: